Oxytetracycline induces anxiety-like behavior in adult zebrafish

Oxytetracycline (OTC) is one of the broad-spectrum antibiotics widely used for treatment fish-farm infection. Considering that behavior directly related to reproduction, individual fitness, and survival, it important evaluate impact on behavioral repertoire in fish. Zebrafish (Danio rerio) presents a well-described reliably demonstrate complex responses chemical compound exposure. This work aims identify role OTC comprehensive parameters whole-body cortisol levels adult zebrafish. Here we report exposure (10, 20, 100 mg/L) induces an anxiogenic-like phenotype novel tank test. also changes social interaction with shoal unknown zebrafish - characterized as stimulus group. exposed (10 remains longer period zone when compared control Clonazepam (0.006 was able reverse induced by OTC. We demonstrated were significantly decreased after mg/L), which not reversed clonazepam. These findings highlight growing utility model understand their underlying mechanisms.